Human Fibroblast Activation Protein (FAP) ELISA Kit

Cat.#:

JL19318

Regulatory Status:

RUO

Alternative:

APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

7.81-500ng/mL

Sensitivity:

3.5ng/mL

Specificity:

This kit recognizes Human FAP in samples. No significant cross-reactivity or interference between Human FAP and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| FAP

Fibroblast Activation Protein (FAP), also termed prolyl endopeptidase FAP, is a membrane-bound serine protease encoded by the FAP gene. The protein structure consists of: (1) an extremely short cytoplasmic N-terminal segment (6 amino acids), (2) a transmembrane domain (residues 7-26), and (3) a substantial extracellular portion containing both an α/β hydrolase domain and an eight-bladed β-propeller domain.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human FAP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human FAP and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	95
1 : 4	Range (%)	89-103	87-108	105-114
1 : 4	Average (%)	94	98	107